Start with the right inputs
Read the labelled mass on the individual vial, not the combined mass across a kit. Enter the final solution volume specified by your laboratory protocol. Final solution volume and solvent added are not automatically the same value in every preparation.
Keep mg, mcg and mL separate
Milligrams (mg) and micrograms (mcg or µg) describe mass; millilitres (mL) describe volume. 1 mg = 1,000 mcg and 1 mL = 1,000 µL. A mass cannot be converted into a volume until the solution concentration is known.
| Input | Equivalent |
|---|---|
| 0.1 mg | 100 mcg |
| 0.5 mg | 500 mcg |
| 1 mg | 1,000 mcg |
| 0.2 mL | 200 µL |
Worked laboratory example
For a specified 10 mg in a final solution volume of 2 mL, the concentration is 10 ÷ 2 = 5 mg/mL. A hypothetical laboratory aliquot containing 1 mg therefore has a volume of 1 ÷ 5 = 0.2 mL, or 200 µL. The 1 mg target is an arithmetic example, not a recommended dose.
What the displayed U-100 scale means
The calculator’s illustration uses a scale with 100 markings per mL: 0.2 mL corresponds to 20 markings on that specific scale. These markings represent volume, not biological potency or peptide mass. Do not apply that relationship to a differently calibrated device. The graphic is a calculation reference, not an injection instruction.
What the calculator cannot determine
It cannot choose a dose, solvent, preparation method, route of administration, storage period or material compatibility. It does not verify the content of a vial. IU-labelled materials cannot be converted into mg using this general mass/volume calculation.
Check these common input errors
Do not enter the total mass of all ten vials when calculating for one vial. Do not enter 500 in the mg field when you intended 500 mcg. Do not confuse a result of 0.2 mL with 2 mL. The calculator rejects non-positive concentration inputs and flags volumes outside the illustrated scale.
For catalog specifications, use the material directory and the guide to strengths and labels.